Platelet Activation in Stored Platelet Concentrates: Comparision of Two Methods Preparation

Preparation conditions and storage of platelet for transfusion may cause platelet activation, which contributes to decreased ability of stored platelet to function and to survive in vivo after transfusion compared with that seen with freshly prepared platelets [1-4]. Platelet transfusion therapy has played an important role in the management of patients [2,4]. Today, platelet concentrates are routinely manufactured from whole blood by differential centrifugation buffy coat-derived platelet concentrates (BC) or by platelet rich plasmaplatelet concentrates (PRP-PC) and plateletpheresis [5-7]. Whereas in 1970 and 1985, the use of BC exceeded that of PRP-PC by far. We here report on the difference between both platelet concentrates in functional properties of platelet in BC and PRP-PC are compared over storage of 5 days. Processing and storage may affect both platelet morphology and function and they may thus be less effective when transfused than fresh platelets [1,3-4,89]. There are several methods for quality control of platelet components including cell counting, pH, swirling and markers for platelet activation [8,10-11]. Redistribution of phosphatidylserine (PS) from the inner membrane surface to the exterior surface accompanies during platelet activation [11,12,13]. The exposure of PS can be measured by using flow cytometry and fluorescent-labeled annexin V. Annexin V was used as a parameter for quality monitoring of platelet concentrates during storage. This essay is based on the principle that annexin V binds to PS, with high affinity and high specificity [8,12-14]. Activation of thrombocytes is followed by an increased CD62P expression; this protein is the main component of the alpha granules secreted from the granules during storage [7-8]. The aim of this study is to characterize the quality of the changes occurred in the platelet activation parameters Corresponding author: Soleimany ferizhandy Ali, Iranian blood transfusion organization-research center, hemmat Exp way, P.O.Box: 14665-1157/Tehran, Iran, Tel: 009888601559; Fax: 009888601559; E-mail: [email protected]